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Human K-ras Gene Mutations Detection Kit

Human K-ras Gene Mutations Detection Kit

Real Time PCR

CE-IVD Certificate no.:MDD-SZ-XW-160321 

For scientific research use in China



Individualized targeted therapy: Using new methods of molecular analysis, to help doctors and patients choose the most effective drugs, and to better control the disease progression according to the patient's genetic characteristics, thus to develop a suitable individualized therapy.


Target Drugs and KRAS Gene


Cetuximab and panitumumab, the target monoclonal antibodies can inhibit EGFR downstream signal transduction. Studies had shown that patients with KRAS mutations will resist cetuximab and panitumumab, FDA indicates in the instruction of cetuximab and panitumumab that such drugs are not recommended to apply to colorectal cancer patients with KRAS mutations.





NCCN clinical practice guidelines in oncology:Non-Small Cell Lung Cancer (Version 4.2016) suggested that pathological assessment should include the detection of KRAS gene mutation, which is one of the reasons for drug resistance. Therefore, KRAS mutation detection is recommanded before taking target drug therapies.


Detection Sites


No.Mutation NameChanges of Amino AcidsChanges of BasesCosmic ID
1Gly12AspGlycine to Asparaginic AcidsGGT>GAT521
2Gly12AlaGlycine to AlanineGGT>GCT522
3Gly12ValGlycine toValineGGT>GTT520
4Gly12SerGlycine to SerineGGT>AGT517
5Gly12ArgGlycine to ArginineGGT>CGT518
6Gly12CysGlycine to CystineGGT>TGT516
7Gly13AspGlycine to Asparaginic AcidsGGC>GAC532



Detection Items



Product

Product

Specification


Matched

Instrument

Detecting

Items

Sample Requirements
Sample TypeQuantity

Preservation

Condition

Human K-ras

Gene Mutations

Detection Kit

10 tests

/kit

StratageneMx3000pTM/3005pTMABI7500/7300 

Detect 7 kinds

of hot spot 

mutations in  

condon12 and

codon13 of 

human K-ras 

gene. 

peripheral

blood

10mL2~6℃
FFPE tissue

7 sections

5-10 μm

room

temperature
pleural effusion50-500mL-20℃±5℃



Detection Process





Advantages and Characteristics


Accuracy and ReliabilityDetection with closed tubes. Require no special treatment of the products.

High SensitivityAccurate detection of single copies of mutated DNA samples as little as10ng genome.

Easy operationOne-step operation. 90 minutes of the whole process.

Good ReproducibilityCan be performed in the general PCR Labs. Results with good reproducibility can be easily obtained without special training.

Advanced Technology: R&D based on independent patented PAP-ARMS technology.