Human MGMT Gene Methylation Detection Kit
Real Time PCR
CE-IVD Certificate no.：No.MDD-SZ-XWA1-180523
Only for scientific research use in China
Chemotherapy Resistance and MGMT Gene Methylation
Human MGMT gene is stably present in all normal tissue cells, and its encoded MGMT protein has the highest activity in human liver, followed by lymph gland and intestinal tract, and the activity of bone marrow cells is the lowest.
As a kind of DNA repair proteins, MGMT can remove the alkyl adduct at O6 locus of guanine from DNA ,which can cause mutagenicity and cytotoxicity , thereby protecting the cells against the damage of the alkylation group.Therefore the function of MGMT is the main reason of cancer’s resistance to the alkylating agent drugs.
Guidelines for Detection Technology of Tumor Individualized Therapy states that only patients with methylation at promoter of MGMT can benefit from temozolomide treatment.
The Significance of Individualized Targeted Drugs
• Help clinicians choose the targeted drugs for the patients.
• Predict drug efficacy and drug resistance.
• Reduce unnecessary treatment to save costs.
Gene Methylation Detection Kit
Detect the methylation status of specific loci
of MGMT gene in
Advantages and Characteristics
Accuracy and Reliability: Detection with closed tubes. Require no special treatment of the products.
High Sensitivity: Accurate detection of single copies of mutated DNA samples as little as10ng genome.
Easy operation: One-step operation. 90 minutes of the whole process.
Good Reproducibility: Can be performed in the general PCR Labs. Results with good reproducibility can be easily obtained without special training.
Advanced Technology: R&D based on independent patented PAP-ARMS technology.