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Human MGMT Gene Methylation Detection Kit

Human MGMT Gene Methylation Detection Kit

Real Time PCR

CE-IVD Certificate no.No.MDD-SZ-XWA1-180523

Only for scientific research use in China


Chemotherapy Resistance and MGMT Gene Methylation 


Human MGMT gene is stably present in all normal tissue cells, and its encoded MGMT protein has the highest activity in human liver, followed by lymph gland and intestinal tract, and the activity of bone marrow cells is the lowest.


As a kind of DNA repair proteins, MGMT can remove the alkyl adduct at  O6 locus of guanine from DNA ,which can cause  mutagenicity and cytotoxicity , thereby protecting the cells against the damage of the alkylation group.Therefore the function of MGMT is the main reason of cancer’s resistance to the alkylating agent drugs.


Guidelines for Detection Technology of Tumor  Individualized Therapy states that only patients with methylation at  promoter of MGMT can benefit from temozolomide treatment.



The Significance of Individualized Targeted Drugs


•  Help clinicians choose the targeted drugs for the patients.

 Predict drug efficacy and drug resistance.

 Reduce unnecessary treatment to save costs.



Detection Items


Product

Product

Specification

Matched

Instrument

Detecting

Items

Sample Requirements
Sample TypeQuantity

Preservation

Condition

Human MGMT 

Gene Methylation Detection Kit

24 tests

/kit

Stratagene

Mx3000pTM/

3005pTM

ABI7500/

7300

Detect the methylation status of specific loci 

of MGMT gene in  

DNA samples.

FFPE tissue

7 sections

5-10 μm

room

temperature



Detection Process





Advantages and Characteristics


Accuracy and ReliabilityDetection with closed tubes. Require no special treatment of the products.

High SensitivityAccurate detection of single copies of mutated DNA samples as little as10ng genome.

Easy operationOne-step operation. 90 minutes of the whole process.

Good ReproducibilityCan be performed in the general PCR Labs. Results with good reproducibility can be easily obtained without special training.

Advanced Technology: R&D based on independent patented PAP-ARMS technology.